Dab Images
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Active energetic bearded grandfather using wireless headphones listening to music. cheerful senior male dancing to radio in bluetooth earbuds, dabbing, making dance move, looking from under his arm
Find & Download Free Graphic Resources for Dab. 600+ Vectors, Stock Photos & PSD files. ✓ Free for commercial use ✓ High Quality ImagesDAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3′-diaminobenzidine)
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Description
The DAB substrate reacts with HRP to yield an insoluble brown-colored product. This kit includes nickel, providing the option of changing the reaction product from brown to gray-black.
Features:
- Ideal for IHC/ICC/ISH and blots
- Heat Stable
- Permanent mounting
- Ideal for single and multiple labeling
- No wait times for mixing and dissolving powders or tablets
- Sufficient reagents to produce 300 ml of working solution
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Peroxidase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Brown, Gray-Black |
| Microscopy | Brightfield, Darkfield, Electron, Spectral Imaging |
| Additional Substrate Properties | Heat Resistant, Multiple Labeling |
| Mounting | Aqueous (Hardening), Non-Aqueous (Permanent) |
| Safety Title | WARNING: Cancer – www.P65Warnings.ca.gov |
Kit Components
Kit Contents:
- 6 ml DAB Reagent 1
- 6 ml DAB Reagent 2
- 6 ml DAB Reagent 3
- 6 ml DAB Reagent 4 (Nickel Optional)
Documents
- IHC Multiplexing Guide
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Datasheet
Product FAQs
I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN ® Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?
1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (
50 µL) of Reagent B only from the VECTASTAIN ® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.
DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. ]]>