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Influence of preoperative supplementation of omega-3 fatty acid in the healing of colonic anastomoses in malnourished rats receiving paclitaxel

Alvo Orlando Vizzotto Junior 1

Antonio Carlos Ligocki Campos 2

Eneri Vieira de Souza Leite Mello 3

Tiago Jacometo Castilho 1

1 Oncology Service, Hospital Santa Rita, Maringá, Paraná State – PR, Brazil

2 Department of Surgery, Universidade Federal do Paraná, Curitiba, PR

3 Department of Morphological Sciences, Universidade Estadual de Maringá (UEM), Maringá – PR

To evaluate the effect of preoperative supplementation of omega-3 fatty acids on the healing of colonic anastomoses in malnourished rats receiving paclitaxel.

we studied 160 male Wistar rats, divided in two groups: one subjected to malnutrition by pair feeding (M) for four weeks, and another that received food ad libitum (W). In the fourth week, the groups were further divided into two subgroups that received omega-3 or olive oil by gavage. The animals were submitted to colonic transection and end-to-end anastomosis. After the operation, each of the four groups was divided into two subgroups that received intraperitoneal isovolumetric solutions of saline or paclitaxel.

mortality was 26.8% higher in the group of animals that received paclitaxel (p = 0.003). The complete rupture strength was greater in well-nourished-oil Paclitaxel group (WOP) compared with the the malnourished-oil Paclitaxel one (MOP). The collagen maturation index was higher in well-nourished-oil saline group (WOS) in relation to the malnutrition-oil-saline group (MOS), lower in malnourished-oil-saline group (MOS) in relation to malnourished-ômega3-saline one (M3S) and lower in the well-nourished-omega3-saline group (W3S) compared with the malnourished-omega3-saline (M3S). The blood vessel count was higher in the malnourished-oil-saline group (MOS) than in the malnourished-oil-paclitaxel group (MOP) and lower in the malnourished-oil-saline group (MOS) in relation to the malnourished-omega3-paclitaxel group (M3P).

supplementation with omega-3 fatty acids was associated with a significant increase in the production of mature collagen in malnourished animals, with a reversal of the harmful effects caused by malnutrition associated with the use of paclitaxel on the rupture strength, and with a stimulus to neoangiogenesis in the group receiving paclitaxel.

Key words: Anastomosis, Surgical; Malnutrition; Omega-3 Fatty Acids; Paclitaxel

avaliar o efeito da suplementação pré-operatória dos ácidos graxos ômega-3 sobre a cicatrização das anastomoses colônicas em ratos desnutridos que receberam paclitaxel.

foram estudados 160 ratos Wistar, distribuídos em dois grupos: um submetido à desnutrição pelo pair feeding (D) durante quatro semanas, e outro que recebeu ração ad libitum (N). Na quarta semana, os grupos foram subdivididos em dois subgrupos que receberam, por gavagem, ácido graxo ômega-3 ou azeite de oliva. Os animais foram submetidos à transecção colônica e anastomose término-terminal. Após a operação, foram distribuídos em dois subgrupos que receberam soluções isovolumétricas de salina ou paclitaxel, intraperitonial.

A mortalidade foi 26,8%, maior no grupo de animais que recebeu paclitaxel (p=0,003). A força de ruptura completa foi maior no grupo nutrido-azeite-paclitaxel (NAP) em relação ao grupo desnutrido-azeite-paclitaxel (DAP). O índice de maturação de colágeno foi maior no grupo nutrido-azeite-salina (NAS) em relação ao grupo desnutrido-azeite-salina (DAS), menor no grupo desnutrido-azeite-salina (DAS) em relação ao grupo desnutrido-ômega3-salina (DOS) e menor no grupo nutrido-ômega3-salina (NOS) em relação ao desnutrido-ômega3-salina (DOS). A contagem do número de vasos sanguíneos foi maior no grupo desnutrido-azeite-salina (DAS) em relação ao grupo desnutrido-azeite-paclitaxel (DAP) e menor no grupo desnutrido-azeite-salina (DAS) em relação ao grupo desnutrido-ômega3-paclitaxel (DOP).

a suplementação com ácidos graxos ômega-3 associou-se ao aumento significativo na produção de colágeno maduro nos animais desnutridos, à reversão do efeito deletério causado pela desnutrição em associação ao uso do paclitaxel, sobre a força de ruptura, e ao estímulo da neoangiogênese no grupo que recebeu paclitaxel.

Palavras-Chave: Anastomose Cirúrgica; Desnutrição; Acido Graxo Ômega-3; Paclitaxel

Ovarian cancer is the second most common gynecological cancer in Brazil, with an annual mortality of about 15,000 cases 1 . It is often diagnosed at a stage when there is peritoneal dissemination, and has surgery as its initial treatment approach with the aim of ideal cytoreductive resection, i.e., residual disease that is less than 0.5cm 2 . To this end, intestinal segments resection and performance of colonic anastomosis are frequently required. Early chemotherapy containing paclitaxel is an important treatment element 3 . The healing process of these colonic anastomosis takes place in an adverse environment, under the effects of malnutrition, which is very common in patients with ovarian cancer with peritoneal dissemination, and of the immunosuppressive effects of chemotherapy 4 .

Preoperative food intake is crucial to ensure that the postoperative healing process will properly occur 4 – 6 . Although preoperative adequate renutrition is difficult in such patients, supplementation with specific nutritional elements in small volumes can be an alternative to accomplish it, especially if the substrate has a nutritional modulatory role in the healing process, enabling it to elapse with smaller effects of malnutrition 7 .

Experimental studies conducted to evaluate the effect of paclitaxel in colonic anastomosis in rats showed that when administered in the postoperative period, this drug hampers the healing process 8.9 . In this scenario, the omega-3 fatty acids have been proposed as a nutritional element that allow caloric intake and favorable modulation of the inflammatory process and healing 4 , 10 , 11 .

This study evaluated the effect of preoperative supplementation of omega-3 fatty acids on the healing of colonic anastomoses in malnourished rats receiving intraperitoneal paclitaxel.

Experimental design and administered solutions

We used 160 male Wistar rats (Rattus norvegicus albinos, Roentia Mammalia) with body weight ranging from 200 to 330 grams (288.76 ± 4,45g) from the vivarium of the Universidade Estadual de Maringá (UEM). The animals were housed in individual boxes and divided into two main groups: W – well-nourished rats (n = 80) who received food and water ad libitum; M – malnourished rats (n = 80) who received half the amount of the diet that their well-nourished pairs had received the previous day (malnutrition process, pair feeding method). Each group was divided into four subgroups with 20 animals each, according to lipid supplementation and the administration of saline or paclitaxel.

The lipid solutions were extra-virgin olive oil emulsion and omega-3 fatty acids consisting of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in a ratio of 80:20 and concentration of 20mg / ml. The administration comprised a single daily dose of 100mg / kg via orogastric gavage without anesthesia.

The drug used was paclitaxel at a dose of 3.5 mg / kg diluted in isotonic saline solution at a concentration of 0.35 mg / ml. The administration of chemotherapy or 0.9% isotonic saline solution was carried out with intraperitoneal injection, immediately after the surgical procedure.

The well-nourished rats (W) received chow ad libitum the four weeks prior to the procedure and were divided into four subgroups, being supplemented with: WOS – olive oil and saline; W3S – omega 3 and saline; WOP – olive oil and paclitaxel; and W3P – omega 3 and paclitaxel. The malnourished rats (M) were divided into four subgroups and were supplemented with: MOS – olive oil and saline; M3S – omega 3 and saline; MOP – olive oil and paclitaxel; and M3P – omega-3 and paclitaxel.

Surgical procedure and evolution

The surgical procedure, conducted under general anesthesia with a combination of 10 mg / kg xylazine and 50 mg / kg ketamine, consisted of two colon transections (colostomies), one at the colon proximal segment, 4.0 cm from the ileocecal valve, and one at the distal colon segment, 3.0 cm above the peritoneal reflection of the rectum. The reconstructions were performed in total single plane end-to-end anastomosis with interrupted suture using eight stitches of 6-0 monofilament, synthetic, nonabsorbable suture (Ethicon?). After anesthetic recovery all animals received food and water ad libitum until the time of death.

We observed and weighed the animals daily, and those which died underwent necropsy to evaluate the presence of fistula or peritonitis. Euthanasia, with a lethal dose of ketamine and xylazine association, was taken on the fifth day after surgery. After death, the animals underwent celiotomy for evaluation of the following: presence of wound infection, adhesions (by the Knigthly index) 12 and anastomotic complications (dehiscence or stenosis). Then we excised the proximal and distal colon segments containing the anastomoses. The proximal segments, about 2.0 cm long, were named proximal specimen (PS) and used for pathological and immunohistochemical study. The distal segments, 4 cm long, were named distal specimen (DS) and subjected to tensile test.

The distal specimen was subjected to tensile test, using a computerized mechanical testing machine (EMIC(r)). Tension was applied with a 50 mm / minute speed. We used a 1Kg load cell with a sensitivity of 50 grams for a 4500 gf strength limit, until complete anastomotic rupture. The variables evaluated were the maximum tensile strength (MTS) and the complete rupture strength (CRS), measured in Newtons (N), and the maximum tension (MT), measured in N / cm 2 .

The proximal specimen was fixed in buffered 10% formalin and sent for histological processing and evaluation by hematoxylin-eosin (HE), picrosirius-red (PSR) and immunohistochemistry.

HE staining was used to assess the degree of inflammatory reaction (poly and monomorfonuclear infiltrate), interstitial edema, vascular congestion, granulation tissue and fibrosis. The data were classified as severe, moderate, mild or absent, and transformed into quantitative variables by assignment of indexes to the histological findings, as follows: absent, index 0; discrete, index 1; moderate, index 2; and severe, index 3.

The staining with F3BA picrosirius-red was made to identify the mature and immature collagen fibers by microscopy technique with polarized light and computerized morphometric analysis. Each section was assessed by optical microscopy at 200x magnification using a polarized light source (C-SP simple polarizer). We analyzed two fields, proximal and distal to the anastomotic line, containing the entire thickness of the colonic segment. The images were captured by a Nikon DS-FI1C camera and transmitted via the D-DA simple system analyzer, to the G205HV(r) color LCD monitor, frozen and scanned. We then performed image analysis using the Image Pro-plus(tm) software. In the RGB (“Red, Green, Blue”) system, we quantified areas of mature collagen fibers – collagen type I (shades of red, yellow and orange) – and immature ones – type III collagen (green tones). We computed the between the area percentages of collagen type I and collagen type III, determined as collagen maturation index (CMI).

By immunohistochemical method, we evaluated CD31 (Monoclonal, JC70 code, Cell Marque, Moleenstraat, NL), calponin (Monoclonal, code CALP, Cell Marque, Moleenstraat, NL) and Ki-67 (Monoclonal, SP6 code, Cell Marque, Moleenstraat , NL), on separate slides. We used these markers to evaluate neoangiogenesis, research for myofibroblasts, and for cells in the replication phase (other than G0) present in the healing process area. We carried out antigen retrieval by exposing the materials to heat and pressure. Development of the slides was made by Diaminobenzidine (DAB) brown chromogen; the detection technique, by HRP-polymer (free biotin). We used positive and negative controls to validate the reactions. We examined the slides under 200x magnification to count vessels and 400x to count myofibroblasts and proliferating cells. The images were made using the pictures OPT 5000 Power Opticam(r) capture system, of 5.0 megapixels, connected to a computer with image analysis software (VMS 3.5 Measuring Software HPower(r)). We performed quantitative analysis of the number of vessels, fibroblasts and proliferating cells in the area of the anastomosis, as well as 10mm proximal and 10mm distal to it, in four randomly selected different fields.

We carried out the statistical analysis according to the nature of the data. We used parametric tests for quantitative variables with normal (Gaussian) distribution. For comparison between all groups, was applied the analysis of variance (ANOVA), and for the pair-wise comparison of groups, we used the Student’s t test. For quantitative variables without normal (Gaussian) distribution, we used non-parametric analysis. For comparison between all groups, we applied the Kruskal-Wallis test, and for pair-wise analysis, the Mann-Whitney test (Wilcoxon test for independent groups). We evaluated dichotomous variables, such as a fatal outcome, presence of wound infection and anastomotic complications (dehiscence or stenosis) by the chi-square test and the Cochran test. The level of significance was p Figure 1 Evolution of animal weight. X-axis: days of the experiment – D1: Day 1; D7: 7th day; D14: 14th day; DOP- day of operation; and DEUT: day of mice death (euthanasia). Y-axis: Weight (grams). WOS: well-nourished-oil-saline; W3S: well-nourished-omega-3-saline; WOP: well-nourished-oil-paclitaxel; W3P: well-nourished-omega-3-paclitaxel; MOS: malnourished-oil-saline; M3S: malnourished-omega-3-saline; MOP: malnourished-oil-paclitaxel; M3P: malnourished-omega-3-paclitaxel.

On the operation day (Figure 2), the average weight of the MOS group (236.5 ± 4.2 g) was significantly lower (p Figure 2 Mean weights in malnourished groups on the day of operation. Y-axis: weight in grams; X-axis: groups: MOS – malnourished-oil-saline; M3S – malnourished-omega-3-saline; MOP – malnourished-oil-paclitaxel; M3P – malnourished-omega-3-paclitaxel; e – Average of MOS group weight is smaller than the average weight of M3S group (p

Anastomotic stenosis occurred in 14 rats of the well-nourished group and in 31 of the malnourished group, being significantly higher in this group (p = 0.007).

Full tensile strength (Figure 3) was significantly higher in the WOP group compared with the MOP group (1.28 ± 0.17 vs. 0.77 ± 0.07, p Figure 3 Complete Rupture Strength (CRS) in groups. Y-axis: force in Newtons. X axis: groups; WOS: well-nourished-oil-saline; W3S: well-nourished-omega-3-saline; WOP: well-nourished-oil-paclitaxel; W3P: well-nourished-omega-3-paclitaxel; MOS: malnourished-oil-saline; M3S: malnourished-omega-3-saline; MOP: malnourished-oil-paclitaxel; M3P: malnourished-omega-3-paclitaxel. There was no significant difference between groups (p = 0.3638).

The mean intensity of the inflammatory infiltrate (Figure 4) was significantly lower in the MOS group compared with the M3S group (1.61 ± 0.78 vs. 2.50 ± 0.63, p Figure 4 Intensity of the inflammatory infiltrate at HE. Y-axis: intensity index of inflammatory infiltrate. X axis: groups. WOS: well-nourished-oil-saline; W3S: well-nourished-omega-3-saline; WOP: well-nourished-oil-paclitaxel; W3P: well-nourished-omega-3-paclitaxel; MOS: malnourished-oil-saline; M3S: malnourished-omega-3-saline; MOP: malnourished-oil-paclitaxel; M3P: malnourished-omega-3-paclitaxel.; a- intensity of the inflammatory infiltrate of the MOS group significantly lower than of the M3S group (p

Regarding the collagen maturation index (CMI) (Figure 5), it was significantly higher in the WOS group than in the MOS one (1.45 ± 0.4 vs. 0.35 ± 0.08, p Figure 5 Collagen maturation index. Y-axis: collagen maturation index (CMI) average. X axis: groups. WOS: well-nourished-oil-saline; W3S: well-nourished-omega-3-saline; WOP: well-nourished-oil-paclitaxel; W3P: well-nourished-omega-3-paclitaxel; MOS: malnourished-oil-saline; M3S: malnourished-omega-3-saline; MOP: malnourished-oil-paclitaxel; M3P: malnourished-omega-3-paclitaxel; a- WOS CMI was significantly higher than MOS (p

The average blood vessels count (Figure 6) was significantly higher in the M3S group compared with the MOP group (12.64 ± 1.7 vs. 6.45 ± 0.28, p Figure 6 Number of blood vessels according to the CD 31 marker. Y-axis: number of vessels. X axis: groups. WOS: well-nourished-oil-saline; W3S: well-nourished-omega-3-saline; WOP: well-nourished-oil-paclitaxel; W3P: well-nourished-omega-3-paclitaxel; MOS: malnourished-oil-saline; M3S: malnourished-omega-3-saline; MOP: malnourished-oil-paclitaxel; M3P: malnourished-omega-3-paclitaxel; a- number of vessels was significantly higher in MOS the group than in MOP (p

The overall incidence of deaths in this study was 26.5%, consistent with other studies using similar methodologies. Trubian observed 24.5% of deaths when assessing malnutrition with toxemia induction 13 . The mortality rate was higher in the groups receiving paclitaxel compared with the groups that received saline, most due to anastomotic postoperative complications.

The immunosuppressive action of chemotherapy drugs such as paclitaxel is one of the possible factors associated with higher death rates observed in the groups receiving this drug 2 , 14 . Another possible explanation for this higher death rate is because such drugs act by inhibiting cell proliferation by different mechanisms of action. As such proliferation is essential in the healing process, they play an important attenuation of the fibroplasia process 8 , 9 , 15 . The inhibitory action on fibroplasia results in higher anastomotic dehiscence rate, causing fistula and fecal peritonitis 15 , which is consistent with what was observed at the animals’ necropsy.

The average starting weight of the animals in all groups was homogeneous. The induction of protein-energy malnutrition, by restriction to 50% of the standard diet, promoted progressive weight loss during the 28 days. The animals in well-nourished groups had an average weight gain of 23.1% between D1 and D28, while the ones in malnourished group had a mean weight loss of 16.6% between D1 and D28. The average weight of the malnourished group was lower compared with the well-nourished ones at the end of 28 days, showing the efficiency of the food restriction procedure on obtaining malnutrition. Similar results were found in other studies 13 , 16 .

Relatively few studies have evaluated the role of supplementation of omega-3 fatty acids in post-anastomosis recovery of the digestive tract, most of them clinical studies. Clinical trials such as the ones of Farreras et al., in 2005 17 , Torrinhas, 2013 4 , and Aoyama et al., in 2013 18 , evaluated the effect of omega-3 fatty acids supplementation in patients undergoing digestive tract anastomosis. Only in the latter, the weight was evaluated as a nutritional recovery indicator, and the omega-3 fatty acids had an important role for nutritional recovery and weight gain in postoperative patients undergoing gastroenteric anastomosis for stomach cancer 18 .

We also observed that in the operation day, after one week of olive oil or omega-3 fatty acids administration, the MOS group mean weight was greater than the M3S group. This finding, consistent with the literature, suggests that supplementation with omega-3, besides favorably modulating the anastomotic healing process, also contributes as an important nutritional energy intake factor in the recovery from undernourishment 18 .

Of the three measured tensile resistance strengths, the maximum tensile strength (MTS) and the maximum tension (MT) did not differ between groups. Concerning the complete rupture strength (CRS), it was lower in the MOP group when compared to the WOP. Malnutrition associated with paclitaxel played a deleterious role on the anastomotic tensile strength. In this sense, experimental studies that evaluated the effect of malnutrition 13 , 16 and the use of chemotherapy 15 on the on the rupture strength of colonic anastomoses in rats also observed its significant reduction due to harmful effects of such drugs on the healing process.

The induction of protein-energy malnutrition interferes systemically in protein synthesis. Possibly, this effect also occurs in the anastomotic site, hampering collagen synthesis. As the synthesis of collagen in the anastomosis is one of the major recovery factors of tensile resistance strength 13 , 19 , disturbances in this synthesis are possibly associated with a reduction in tensile strength. This mechanism might explain the finding of reduced complete rupture strength (CRS) in the MOP group in relation to the WOP one. The also lower collagen maturation index (ratio between mature and immature collagen) in the MOP group when compared with the WOP, although not significant, corroborates this idea.

The action of chemotherapy on cell proliferation attenuates the fibroplasia process 8 , 9 , 15 . Possibly this is an enhancer element of the malnutrition inhibitory effects on protein synthesis, especially collagen in the anastomotic site. These elements, malnutrition and paclitaxel administration, acting concurrently, probably explain the reduction of CRS in the MOP group in relation to the WOP group, as this reduction was not observed in the group of animals submitted only to malnutrition without paclitaxel administration (MOS) in relation to the WOS group.

On the other hand, there was no significant difference in CRP between the M3P and W3P groups. This finding suggests that nutritional supplementation with omega-3 fatty acids can reverse the deleterious effects caused by the association of malnutrition and paclitaxel administration in colonic anastomosis CRS, for the significant reduction of this force, observed in the MOP group in relation to the WOP one, was not observed when the M3P group was compared with the W3P group. This finding is consistent with the ones of Ekçi et al. 20 , who also observed a significant increase in the colonic anastomosis rupture pressure of rats receiving dietary supplementation with omega-3, although used in combination with ascorbic acid. The favorable modulation of omega-3 on collagen synthesis in the anastomotic site is one possible explanation for the observed recovery of the colonic anastomosis rupture strength.

We also noted an increase in cell activity in the anastomotic site, considered as inflammatory infiltrate, in the M3S group compared with the M3S one. This finding reinforces the idea that omega-3 has immunomodulating effects in inflammation and healing, by stimulating cellular activity, and corroborates the findings of McDaniel et al. 21 , who observed an inflammatory infiltrate increase of human healing skin wounds, as well as a significantly higher level of interleukin 1â (IL-1 â) in the inflammatory lesion fluid in the group that received the administration of EPA and DHA. This finding may seem conflicting with that proposed in the literature, that the omega-3 fatty acids have less pro-inflammatory activity by replacing arachidonic acid (omega-6 fatty acid) in the cell membrane and increase, through its metabolism, the production of eicosanoids of lower inflammatory potential, such as leukotriene B5 and prostaglandin E3 10 , 22 . Authors speculate that this increased cellular activity of the anastomotic environment is due to specific cell types, such as macrophages, fibroblasts, and myofibroblasts. It is possible that at the anastomotic site eicosanoids derived from the omega-3 fatty acids, although display an inhibitory effect on the inflammatory process in general, can stimulate the proliferation of specific cell types. The largest production of mature collagen (CMI) in the M3S group compared with the M3S one contributes indirectly to this idea, as this increased production is correlated with increased activity of cells producing collagen (fibroblasts and myofibroblasts) in the anastomotic environment.

The evaluation of collagen densitometry showed a significant reduction of mature collagen (type I) in relation to the immature one (type III), when assessed by the collagen maturation index (CMI), in the MOS group compared with the WOS group. This finding suggests that malnutrition is an important factor reducing the production of mature collagen in the healing anastomosis. Trubian also observed a reduction of CMI in groups of malnourished rats compared with the group of well-nourished ones 13 .

The recovery of the tensile strength is more related to type I collagen than with type III one. The deposition and remodeling of type I collagen fibers is the main factor for recovery of anastomotic resistance 19 .

The M3S group showed an important gain of mature collagen in relation to the immature one when compared to W3S group, as the CMI of the former was greater than the one of the latter. This finding suggests that the administration of omega-3 in the week before the surgical intervention stimulates the production of mature collagen in malnourished animals. The CMI was also significantly higher in the M3S group compared with the MOS one. This finding supports the idea that preoperative administration of omega-3 can reverse the deleterious effects of malnutrition in the production of mature collagen in the healing anastomotic environment.

The migration and proliferation of fibroblasts and myofibroblasts in the healing environment, and consequently the production of collagen, is mediated by the action of inflammatory prostanoids (prostaglandins and leukotrienes) produced by inflammatory cells. Therefore, the higher CMI in the M3S group in relation to the MOS is possibly correlated with the higher level of cellular activity observed in the M3S group compared with the MOS group.

Another finding of this study that favors the concept of immunomodulation produced by omega-3 was the observation made in the number of blood vessels (neoangiogenesis) in the anastomotic healing environment. In immunohistochemistry, CD31 is mainly used to demonstrate the presence of endothelial cells in histologic tissue sections. This allows evaluating the degree of tissue angiogenesis 23 . We observed a decrease in the number of blood vessels in the MOP group in relation to the MOS group, suggesting that the administration of paclitaxel, which is a drug with antiangiogenic action 24 , is the causative factor of such reduction, agreeing with another study 8 that showed significant reduction in neovascularization of colonic anastomoses of rats receiving paclitaxel. Supplementation with omega-3 in the M3P group was associated with recovery of the number of vessels, since this number was higher in this group compared with the MOP group. This finding suggests that the inhibition that paclitaxel causes on neoangiogenesis of the colonic anastomosis healing process can be reversed with the addition of omega-3 preoperatively. It is speculated that the eicosanoids produced by the metabolism of omega-3 fatty acids, such as prostaglandin E3 and leukotriene B5, stimulate the migration and proliferation of endothelial cells precursors in the anastomotic environment.

Myofibroblasts are (predominantly) type I collagen-producing cells, and its quantification can also be used as a healing evaluation method 13 . Fibroblasts start to appear in the wound around the third day after surgery, proliferate in response to growth factors and begin to produce collagen 25 . We observed a significant reduction in the average number of myofibroblasts in the M3S group compared with the W3S one. This finding suggests that malnutrition is a deleterious effect to the myofibroblasts production. Trubian also found a significant reduction in the number of myofibroblasts in colonic anastomosis in malnourished rats 13.

Proliferating cells are the very hallmark of anastomotic healing. The Ki-67 antigen is present on all active phases of the cell cycle (G1, S, G2 and mitosis), but is absent on resting cells (G0) 26 . In this study, there was no difference between groups in the number of proliferating cells when assessed by Ki67. Given the previously reported findings and hypotheses developed, one would expect to find more proliferating cells in the groups receiving omega-3 fatty acids, particularly in subgroups that did not receive chemotherapy, when compared with groups receiving olive oil, but we did not. We could not establish objective reasons for this. One suggested possibility is that proliferating cell types are different between the groups olive oil and omega-3 fatty acids. In the latter, with healing mediated by eicosanoids of lower inflammatory potential (prostaglandin E3 and leukotriene B5) derived from the metabolism of omega-3 fatty acids, cell types would be composed of myofibroblasts, fibroblasts, endothelial cells and their precursors; in the former group (olive oil), with healing mediated by prostanoid of more inflammatory potential, other cell types would prevail, like polymorphonuclear and mononuclear ones.

In agreement with the literature, the data presented here suggest that the use of omega-3 was associated with modulation of the inflammatory process, with stimuli to collagen production and to its quality in the anastomotic environment 19 . As already mentioned, the amount of mature collagen in relation to the immature, as measured by the CMI, was significantly higher in the M3S group compared with the MOS. Also in the M3S group, cellular activity represented by the inflammatory infiltrate in the anastomosis was higher than in the M3S group. These two concomitantly assessed findings support the idea that preoperative omega-3 administration in malnourished animals contributes positively to the healing process modulation of colonic anastomoses in rats, corroborating data from another work 20 , which pointed to the beneficial action of fatty acids.

The supplementation of omega-3 was also an important energy intake nutritional factor, as it attenuated weight loss in the M3S group compared with the MOS. This supplementation also played an important role in the recovery of the deleterious effects of paclitaxel on neoangiogenesis, since the number of blood vessels in the M3P group was higher than in the MOP one.

The exact mechanisms by which omega-3 may have acted to produce this study’s findings still need to be clarified. Omega-3 fatty acids have immunomodulatory activities, still under study, on the production of cytokines, activation of T lymphocytes and angiogenesis 27 , which, when understood, can contribute to a better data comprehension.

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Omega dab Influence of preoperative supplementation of omega-3 fatty acid in the healing of colonic anastomoses in malnourished rats receiving paclitaxel Alvo Orlando Vizzotto Junior 1 ]]>