DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3′-diaminobenzidine)
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Description
The DAB substrate reacts with HRP to yield an insoluble brown-colored product. This kit includes nickel, providing the option of changing the reaction product from brown to gray-black.
Features:
- Ideal for IHC/ICC/ISH and blots
- Heat Stable
- Permanent mounting
- Ideal for single and multiple labeling
- No wait times for mixing and dissolving powders or tablets
- Sufficient reagents to produce 300 ml of working solution
Specifications
| Unit Size | 1 kit |
|---|---|
| Applications | Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot |
| Detection Enzyme(s) | Peroxidase |
| Substrate Reaction Product | Precipitating Reaction Product |
| Chromogen Color | Brown, Gray-Black |
| Microscopy | Brightfield, Darkfield, Electron, Spectral Imaging |
| Additional Substrate Properties | Heat Resistant, Multiple Labeling |
| Mounting | Aqueous (Hardening), Non-Aqueous (Permanent) |
| Safety Title | WARNING: Cancer – www.P65Warnings.ca.gov |
Kit Components
Kit Contents:
- 6 ml DAB Reagent 1
- 6 ml DAB Reagent 2
- 6 ml DAB Reagent 3
- 6 ml DAB Reagent 4 (Nickel Optional)
Documents
- IHC Multiplexing Guide
- Enzyme Substrates Brochure
- Enzyme Substrate Combinations Chart
- Counterstain/Substrate Compatibility Chart
- User Guide
- Safety Data Sheet
- Datasheet
Product FAQs
I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN ® Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?
1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (
50 µL) of Reagent B only from the VECTASTAIN ® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.
DAB (3, 3 -diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications.
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- ImmunoDetection
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DAB Peroxidase (HRP) Substrate Kit (with Nickel), 3,3’-diaminobenzidine
- Supplier: Vector Laboratories
Special Offer: Purchase 1 Vector Product at List Price and Receive a 50% Discount off the List Price of a Vector Product You’ve Never Tried Before! Reference Promotion Code VECT20NEW. Valid until November 30, 2020. 50% discount applies to a product of equal or lesser value that has not been previously purchased by the customer. Cannot be combined with other offers or discounts.
Product Description
- Greater sensitivity than conventional substrates
- Consistent and reliable
- Ideal for IHC/ICC/ISH and blots
- Heat Stable
- Permanent mounting
- Ideal for single and multiple labeling
- Stock solutions supplied in convenient dropper bottles promoting ease of handling
- No wait times for mixing and dissolving powders or tablets
- Includes nickel to produce a gray-black rather than brown reaction product
- Sufficient reagents to produce 300 ml of working solution
Resources
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MJS BioLynx Inc.
MJS BioLynx Inc. is a Canadian distributor of quality laboratory products for use in life science research. We offer tools for genomics, proteomics, microbiology, cell biology, sample storage, and more.
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Vector Laboratories Contact Us Toll Free 1-888-593-5969 Technical Support BioLynx Well Being Products ImmunoDetection ICC DAB Peroxidase (HRP) Substrate Kit